Python Md5 Hashing, trailing newline when reading file

I am experiencing unexpected behavior with file-reading and hashing (in Python 3.7). I have a file that simply has the text "helloworld" in it, without a newline at the end:

import hashlib

def hashit(inp):
    return hashlib.md5(inp.encode('utf-8')).hexdigest()

from_var = 'helloworld'

with open('input.txt', 'r') as fo:
    from_file = fo.read()

print(f' from_file      : { repr(from_file) }')
print(f' from_var       : { repr(from_var) }')

print(f' from_file hash : { hashit(from_file) }')
print(f' from_var  hash : { hashit(from_var) }')

helloworld